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yfmL [2019-07-25 08:49:40]
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yfmL [2019-07-25 08:49:40]

DEAD-box RNA helicase
Locus
BSU_07430
Isoelectric point
9.60
Molecular weight
42.03 kDa
Protein length
376 aa Sequence Blast
Gene length
1131 bp Sequence Blast
Function
RNA helicase
Product
DEAD-box RNA helicase
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → RNA synthesis and degradation → DEAD-box RNA helicases
  • Information processing → Protein synthesis, modification and degradation → Translation → Ribosome assembly

    • Gene

    Coordinates
    816,113 817,243

    Phenotypes of a mutant

  • poor growth at low temperatures (16 to 20C) PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • RNA helicase
  • required for ribosome assembly PubMed

    • Protein family

  • helicase family (according to UniProt)
  • DEAD-box RNA helicases (according to UniProt)

    • Paralogous protein(s)

  • CshB, CshA, DeaD

    • Structure

  • 5IVL (CshA, 87% coverage, 35% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    yfmL
    Description
    PubMed

    Regulation

  • expression declines in the stationary phase PubMed
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    Biological materials

    Mutant

  • MGNA-C239 (yfmL::erm), available at the NBRP B. subtilis, Japan
  • GP1053 (yfmL::erm), available in the Jörg Stülke's lab PubMed
  • BKE07430 (yfmL::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTGTTCTCCTCAATTC, downstream forward: _UP4_TAAAAAGAAGGGCGAAGAGC
  • BKK07430 (yfmL::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTGTTCTCCTCAATTC, downstream forward: _UP4_TAAAAAGAAGGGCGAAGAGC

    • Expression vectors

  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1069 (spc), available in Jörg Stülke's lab

    • GFP fusion

  • B. subtilis GP1082 yfmL-yfp spc, available in Jörg Stülke's lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed

    • FLAG-tag construct

  • GP1066 (spc, based on pGP1331), available in Jörg Stülke's lab

    • References

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